Sure, you can always measure in-room and if you want to know what to equalize for, that's important information. One extra tidbit to consider is that in-room response is different at different places in the room. Equipment setup, room treatments and EQ all depend on what areas in the room are desired to have best response.When making measurements for loudspeaker comparison, as the 1W/1M SPL figure is generally applied, tests should be made in an anechoic environment. Measurements made outdoors or in an anechoic chamber are used for comparing loudspeakers, since they remove environmental influence.